Cardiology and Cardiovascular Therapeutics

Introduction: Diabetes mellitus (DM) is a chronic endocrine disorder characterized by hyperglycemia with impaired metabolism of carbohydrates, fats and proteins resulting from disorders of insulin hormone secretion, insulin function, or both. Hyperglycemia and other metabolic changes can cause interference with the production of nitric oxide (NO). Nitric Oxide is synthesized from L-Arginine by endothelial nitric oxide synthase (eNOS) encoded by the eNOS3 gene on chromosome 7. An important polymorphism described in the literature is that G894T results in accelerated degradation and significantly reduces NO production which contributes to the occurrence of atherosclerosis. 

Purpose: Proving the relationship between Nitric Oxide gene polymorphism and Nitric Oxide levels in type 2 DM with hypertension in the Bataknese. 

Method: A Case ontrol study was performed to evaluated the association between polymorphism of G894T Gene and nitric oxide level. 56 consecutiv patients with type 2 diabetes with hypertension and 56 healty controls were recruited. The Genotypes polymorphism were determined by the polumerase chain reacion-restriction fragment length polymorphism (PCR-RFLP) analysis. 

Result: The Polymorphism of G894T Gene Nitric oxide synthase 3 was found to be assosiated with hypertension in type 2 diabetes in the bataknese population as the patients group had higher frequeny compared with the controls. The nitric oxide levels in type 2 diabetes with hypertension in the Bataknese was lower than controls. 

Conclusion: The result of this study indicated that the G894T polymorphism in NOS 3 may be genetic susceptibility factor for hypertension in the bataknese population.